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For the most consistent and reliable SBEM preparation, the PELCO BioWave® Pro+ is the answer.
From tissue to block in less than 6 hours!
These clear, styrene storage boxes contain a dense, closed cell foam insert that have slots cut out. The slots can be used to hold pins such as the Cryo Specimen Pins (#16948, 16949) or Gatan 3View Pins (#16950-1, 16950-3).
Pins shown are sold separately.
Angled serrated tips provide excellent grip in the grooved area of the Serial Block-Face Pin Stub (#16145) above.
Excellent for sample preparation of specimens which are difficult to orient. The tips easily and quickly attach to plastic cylinders. When tip is mounted it is identical in dimensions to a BEEM® 00 size capsule. Blue silicone rubber. Maximum temperature: 218°C (425°F). See our Cyanoacrylate Fast-Glue and Mounting Cylinders.
Number of Cavities: 30 Numbering: OUTSIDE of Cavities, 1-30 Mold Size: 61 x 72 x 6mm thick
for embedding larger specimens, uses less resin
With this mold a larger specimen may be placed in a wider tip area resulting in a larger initial section. Once solidified the block may be glued to the end of a plexiglas rod or other holding device. (See Mounting Cylinders.) Blue silicone rubber. Maximum temperature: 218°C (425°F).
Number of Cavities: 30 Numbering: Outside of cavities, 1-30 Block Faces: 3 x 3mm Mold Size: 61.5 x 72 x 6mm thick
ERL 4221 has replaced ERL 4206. ERL 4221 has a higher viscosity than the previously used ERL 4206.
Spurr AR, 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruc Res, 26, p. 31.
Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, V 14, No 4, July 2006
The Spurr Kit gives excellent penetration for tough materials particularly in the studies of botanical, mineral and other hard biological specimens. When the mixture is kept at room temperature for 24 hours its viscosity will increase to 140cP, as is the case with other resins. When fully formulated, the Spurr’s resin will store in a deep-freeze for two months and at room temperature for two days. Curing is done at 70ºC for about 8 hours (see our Microwave system for significant time saving during epoxy processing).
PELCO® Technical Notes for Low Viscosity Kit (1.54MB PDF)
a reliable conductive ground path for SEM specimens Air drying silver paint to make effective ground. Curable at room temperature and has high adhesion to any material. Surfaces do not have to be prepared prior to application and will adhere to such materials as polymer (phenolic) boards, ceramic, glass, metal, plastic and fiberglass. It should be mixed well with a spatula before using. RoHS compliant. The container is offered in two sizes (15g and 30g) and is provided with an applicator brush. Silver flake grain size is an average of 80%, <1.0µm. Sheet resistance is 0.02 ohms per square @ 1 mil (0.001” = 0.025mm) thickness and is a function of the coating. Service temperature range is -40°C to 200°C. Use #16021 gold/silver thinner/extender to dilute when needed. Technical Notes, PELCO® Colloidal Silver, Product Numbers. 16031, 16034 (417KB PDF) F, I - 16031, 16034 SDS (153KB PDF) F, I - 16021 SDS (PDF 147KB)
Conductive Adhesives Comparison Table
Strong, highly conductive epoxy for solderless connections and repairs of circuit boards, static discharge shielding, grounding and conductive paths. Cure Schedule (Minimum bond temperature/time) 65 to 121°C (150 to 250°F) for 5 to 10 min. 25°C (75°F) for 4 hours Properties:
Service temperature range -91°C to 100°C (-132°F to 212°F) permanent bond. 0.4 ohms/sq/mil (25µm)
Technical Notes, Silver Conductive Epoxy(40KB PDF) 16043 SDS Part A (119KB PDF) I - 16043 SDS Part B (122KB PDF) Conductive Adhesives Comparison Table
Versatile, cost effective SEM pin mount storage box and holder for pin mounts with a ø3.2mm (1/8") pin. Numbered 1-18 with the spacing designed to accommodate all types of pin mounts with a diameter from 6.4 to 50mm. Can hold 18 of 12.7mm pin mounts, 8 of 18mm pin mounts, 8 of 25mm pin mounts, 2 of 32 or 38 or 50mm pin mounts with space left for the smaller pin mounts. Maximum specimen height between mount surface and closed lid is 16mm (5/8"). Used for FEI/Philips, ZEISS/LEO/Leica, Cambridge CamScan, TESCAN, Amray and ETEC SEMs. Box inside dimensions are: 4-5/8" x 2-2/4" x 1-5/16" (11.7 x 7.0 x 3.3cm).
Very strong and rugged clear polycarbonate box to store standard pin mounts with 3.2mm (1/8"). This box is watertight, crushproof and dustproof. The strong hinges, locking clasps and soft silicone O-ring seal makes this an ideal transport, shipping and storage box for SEM samples on pin mounts. The holder insert is numbered 1-18 and accommodates all types of pin mounts from 6.4 to 50mm diameter. Same storage capacity as #16709.
Clear styrene hinged storage box with closed cell rubber foam which holds up to 135 of ø12.7mm (1/2") pin mounts. Foam is glued to the base of the box. Complete with latched and hinged lid. There is a storage map provided with the box. Internal dimensions are 267 x 157 mm ( 10-1/2" x 6-3/16") with a maximum sample height of 24mm (15/16") on the pin stub.
The durable and versatile PELCO® SBEM Pin Stub Vacuum Desiccator holds up to 12 standard ø12.7mm (1/2") pin stubs, or two ø25mm (1") or 1 up to ø50mm (2") SEM pin stubs. Storage under vacuum eliminates contamination or dust. Allows for a 10mm (3/8") specimen height on the pin stubs. The use of durable, non-outgassing, blue anodized aluminum and Silicon O-rings makes it suitable for pumping down to 10-3 mbar. Capable of holding vacuum for many weeks. Metal vacuum valve with PTFE shaft seal. Use a small vacuum pump to evacuate. Vacuum connection for pump-down is a 1/4" (6.35mm) barb fitting.
Overall size w/o vacuum valve is ø76 x 35mm (3" x 1.4"). Cavity height is 13.7mm (0.54"). Also available as a TEM Grid Vacuum Desiccator #16178 or Vacuum Desiccator for Hitachi M4 Stubs #16179-4
PELCO® Technical Notes, PELCO® SEM Pin Stub Vacuum Desiccator (103KB PDF)