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DiATOME® Diamond Knives Overview
Cleaning Tools for DiATOME® Diamond Knives

Now available from Ted Pella, Inc.

DiATOME® cryo Diamond Knives

Diamond knives for low temperature EM and LM applications

Available for sale in U.S.A. only


cryo 25°

The 25° angle of the cryo 25° diamond knife produces sections of frozen hydrated specimens with the least compression and the best structural preservation possible. It is recommended that sectioning be done in a low humidity glove box and the sections should be attached by electrostatic force. Section thicknesses range from 30 to 150nm. Triangular holder. Blade width, 3.0mm.

cryo 35°

The cryo 35° knife has demonstrated its usefulness as a standard knife for the low temperature sectioning of polymers, rubber, paints, etc. The 35° angle represents a balanced compromise between section quality and durability. Two styles (wet and dry) are available. Mounted in the triangular holder cryo 35° is suitable for dry cryo sectioning. The 35° knife mounted in the trough are used for sectioning with fluids such as a DMSO/water mixture. Section thicknesses range 30 – 300nm. Triangular holder (dry) or small boat (wet). Blade widths 1.5 to 4.0mm.

cryo 45°

The cryo 45° knife is well suited for routine cryo sectioning. Like the cryo 35° it is available in two styles (wet and dry). Mounted in the triangular holder cryo 45° is suitable for dry cryo sectioning. The 45° knife mounted in the trough are used for sectioning with fluids such as a DMSO/water mixture. Section thicknesses range 30 to 300nm. Triangular holder (dry) or small boat (wet). Blade widths 1.5 to 4.0mm.

Cleaning Procedure for DiATOME® cryo Diamond Knives
Handling & Use of DiATOME® Diamond Knives (500KB PDF)

Prod # Description Unit Price Order / Quote
Cryo Dry, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK2-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 25°, Knife 3.0mm each P.O.R.
Qty:
123-DK2-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 25°, Knife 3.0mm, Resharpen each 2900.00
Qty:
123-DK3-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm each 2195.00
Qty:
123-DK3-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Exchange each 1550.00
Qty:
123-DK3-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Resharpen each 1190.00
Qty:
123-DK3-CD18R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.8mm, Resharpen each 1500.00
Qty:
123-DK3-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm each 2700.00
Qty:
123-DK3-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK3-CD20R DiATOME® cryo Diamond Knife, Dry Angle 35°,, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK3-CD21R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.1mm, Resharpen each 1650.00
Qty:
123-DK3-CD24R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.4mm, Resharpen each 1900.00
Qty:
123-DK3-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm each 2800.00
Qty:
123-DK3-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Exchange each 1940.00
Qty:
123-DK3-CD25R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK3-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Exchange each 2600.00
Qty:
123-DK3-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Resharpen each 2300.00
Qty:
123-DK3-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm each P.O.R.
Qty:
123-DK3-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Exchange each 2900.00
Qty:
123-DK3-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Resharpen each 2700.00
Qty:
123-DK3-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm each P.O.R.
Qty:
123-DK3-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK3-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Resharpen each P.O.R.
Qty:
123-DK4-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm each 2195.00
Qty:
123-DK4-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Exchange each 1550.00
Qty:
123-DK4-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Resharpen each 1190.00
Qty:
123-DK4-CD18R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.rmm, Resharpen each 1500.00
Qty:
123-DK4-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm each 2700.00
Qty:
123-DK4-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK4-CD20R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK4-CD21R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.1mm, Resharpen each 1650.00
Qty:
123-DK4-CD24R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.4mm, Resharpen each 1900.00
Qty:
123-DK4-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm each 2800.00
Qty:
123-DK4-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm, Exchange each 1940.00
Qty:
123-DK4-CD25R DiATOME® cryo Diamond Knife, Dry, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK4-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm each P.O.R.
Qty:
123-DK4-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Exchange each 2600.00
Qty:
123-DK4-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Resharpen each 2300.00
Qty:
123-DK4-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm each P.O.R.
Qty:
123-DK4-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Exchange each 2900.00
Qty:
123-DK4-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Resharpen each 2700.00
Qty:
123-DK4-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm each P.O.R.
Qty:
123-DK4-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK4-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Resharpen each P.O.R.
Qty:

Prod # Description Unit Price Order / Quote
Cryo Wet, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK3-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm each $2195.00
Qty:
123-DK3-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Exchange each 1550.00
Qty:
123-DK3-CW15R DiATOME® Ccryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Resharpen each 1190.00
Qty:
123-DK3-CW18R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.8mm, Resharpen each 1500.00
Qty:
123-DK3-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm each 2418.00
Qty:
123-DK3-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK3-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK3-CW21R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.1mm, Resharpen each 1650.00
Qty:
123-DK3-CW24R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.4mm, Resharpen each 1900.00
Qty:
123-DK3-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm each 2800.00
Qty:
123-DK3-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Exchange each 1940.00
Qty:
123-DK3-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK3-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Exchange each 2600.00
Qty:
123-DK3-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Resharpen each 2300.00
Qty:
123-DK3-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm each P.O.R.
Qty:
123-DK3-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Exchange each 2900.00
Qty:
123-DK3-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Resharpen each 2700.00
Qty:
123-DK3-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm each P.O.R.
Qty:
123-DK3-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK3-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Resharpen each P.O.R.
Qty:
123-DK4-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm each 2195.00
Qty:
123-DK4-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Exchange each 1550.00
Qty:
123-DK4-CW15R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Resharpen each 1190.00
Qty:
123-DK4-CW18R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.rmm, Resharpen each 1500.00
Qty:
123-DK4-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm each 2700.00
Qty:
123-DK4-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK4-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK4-CW21R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.1mm, Resharpen each 1650.00
Qty:
123-DK4-CW24R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.4mm, Resharpen each 1900.00
Qty:
123-DK4-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm each 2800.00
Qty:
123-DK4-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Exchange each 1940.00
Qty:
123-DK4-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK4-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm each P.O.R.
Qty:
123-DK4-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Exchange each 2600.00
Qty:
123-DK4-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Resharpen each 2300.00
Qty:
123-DK4-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm each P.O.R.
Qty:
123-DK4-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Exchange each 2900.00
Qty:
123-DK4-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Resharpen each 2700.00
Qty:
123-DK4-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm each P.O.R.
Qty:
123-DK4-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK4-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Resharpen each P.O.R.
Qty:





DiATOME® Cleaning Procedure for Cryo Knives

This procedure serves for the cleaning of our cryo immuno and cryo 25° knives. We are at your disposal for any further assistance you might require.

1. Remove the knife stage from the cryo
chamber, leave the knives in the stage,
wash under tap water for warming up.
2. Bevel a polystyrene stick with a
perfectly clean razor blade.
3. Clean the knife with 50% ethanol,
keep wet.
     
 
4. Rinse with 100% ethanol. 5. Dry with a dustblower or canned air.