About The Science:
Value is “Y” or “N”. This field indicates that the article specifically contained the results of a experiment regarding microwave use, and not simply a use of the microwave in a paper around another purpose. This is a searchable field.
Author(s):
If this is a journal article, these are the authors of the article. If this was a book chapter, these were the authors of the chapter but not necessarily the editor of the book. You can search on any name in this field.
Title:
If this was a journal article, this was the title. If this was a book chapter, it was the title of the book chapter, including the chapter number. You can search on any word or phrase in this field.
Author(s) Who Used The PELCO BioWave®/Microwave
If the author who used the Biowave is known, their name is stated. Often this is not known. This is a searchable field.
Author Email(s):
This is the author to contact regarding questions about the published material. This is a searchable field.
Primary Author Institution:
In published works there can as many institutions as there are authors, and often even more since some authors belong and are funded by more than one institution. Nevertheless, the contents of this field is usually limited to either the institution of the Contact Author or the Author who used the Biowave. To find all the institutions, go to the published work. This is a searchable field.
Year:
The year the material was published. The year can be searched.
Keywords:
If the published material contains a keyword list, that list is posted. Not every published work contains a list of keywords. Any keyword can be searched.
Journal:
If the published material came from a journal, it is stated here. Not all material came from journals. This is a searchable field.
Specific Journal Reference:
This contains the volume information for a journal, as well as issue and page range. This is only a search result and is not a searchable field. A page range might also come from a chapter in a book.
Page(s) where PELCO BioWave® / Microwave is Discussed :
This is the page or pages where a reference to microwave/Biowave use is noted. This could be either from a journal or a book. This is only a search result and is not a searchable field.
In Book:
If the published material came from a book, the book authors/editors are stated here. This is a searchable field.
Book Authors / Editors:
If the published material came from a book, the book authors/editors are stated here. This is a searchable field.
Model PELCO BioWave® / Microwave Used:
If the model of microwave/Biowave that was used is known, it’s stated here. This may be important in regards to the features and functionality available to the researcher as to what the instrument was capable of performing regarding tissue processing. This is a searchable field.
PELCO® SteadyTemp™?
The value of this field is “Yes” or “No”, whether a PELCO® SteadyTemp™ was used in any step of the process. This is a searchable field.
Type of Organism(s) Processed:
This is singular in number, even if multiple specimens were used. It is the species of organism used in the experiment. There could be multiple values in this field. This is a searchable field.
Organism Domain / Kingdom:
This is a more general breakdown of the type of organisms processed. It will primarily be usable to find plants (Kingdom “Plantae”) and animals (Kingdom “Animalia”), but the valid Kingdoms that might be searched are:
Animalia
Archaea
Bacteria
Chromista
Eukarya
Fungi
Plantae
Protists
Viriae
“Viriae” are included as a placeholder for viruses, since the taxonomic classification of viruses is not settled.
Some types may not yet have research work where a PELCO® Microwave Tissue Processor was used.
Type of Imaging:
Valid values are:
Array Tomography
Brightfield Microscopy
Confocal Microscopy
Differential Interference Contrast Microscopy
Electron Tomography
FIB-SEM
Fluorescence In Situ Hybridization
Fluorescence Microscopy
Fluorescent microspectroscopy
Light Microscopy
Light Microscopy, Histology
micro-CT
nanoSIMS
RNAscope ISH
Scanning Electron Microscopy
Transmission Electron Microscopy
Tissue Processing Step(s)
If any step in a tissue processing protocol used a microwave, it is mentioned here. A specimen processing step will only be mentioned once, although it’s common for steps to be repeated throughout a process. This is a searchable field.
Certain terms mean something similar or the same, but have been left as they were used in the article, although efforts were made to standardize as much as possible (such as Post-Fixation v. Secondary Fixation; Rinse v. Wash)
Valid values are:
Antibody
Antigen Retrieval
Blocking
Buffer Wash
Clearing
Conjugation
Cryoprotection
Decalcification
Dehydration
Deparaffinization
Drying
Etching
Fixation
Freeze Substitution
Incubation
Infiltration
Immunohistochemical Labeling
Immunocytochemistry: Primary Incubation
Immunocytochemistry: Secondary Incubation
Immunohistochemistry: Primary Incubation
Immunohistochemistry: Secondary Incubation
Immunolabeling: Primary Incubation
Immunolabeling: Secondary Incubation
Paraffin Embedding
Polymerization
Post-Fixation
Quench
Rinse
Secondary Fixation
Silver Enhancement
Slide Drying
Staining
Substrate
Wash
Type of Resin:
If the microwave was used to infiltrate the specimen with resin, and/or polymerize the resin block, the value of this field is “Yes”; otherwise the value of this field is “No”, even if the specimen was infiltrated or polymerized in resin but without using a microwave. This is a searchable field.
Use of Vacuum:
The valid values in this field are “Yes” and “No”. If the value is “Yes”, this means that at least one processing step used vacuum. This is a searchable field.
IHC:
If a microwave was used for an immunohistochemical step in the protocol, the value of this field is “Yes”; otherwise it’s “No”. This is a searchable field.
Immunogold:
If a microwave was used for an immunogold step in the protocol, the value of this field is “Yes”; otherwise it’s “No”. This is a searchable field.