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Cargille Microscope Immersion Oils

for light microscopy, fluorescence microscopy, inclined instruments and projection equipment

Cargille Immersion Oils are in full accord with the FDA requirements. To date, no other immersion oils meet the FDA criterias. Cargille Immersion Oils are PCB (polychlorinated biphenyls) free.

Ted Pella, Inc. offers the four standard types of immersion oils:
Type A, Type B, Type NVH and Type 300 (in 5- pack).

Which Type - Which Application

For Normal Light Microscopy
Type A & B, with viscosities of 150 and 1250 cSt (centistokes), respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, Type NVH or VH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.

For Horizontal, Inverted & Inclined Instruments and Projection Equipment
Higher viscosities are generally required. The new Type NVH meets this requirement, having 21,000 cSt.

For Fluorescence Microscopy
A low fluorescence oil that has been widely used for many years is PCB-Free Type A, and for many applications, this is the recommended type.

Optical and Physical Properties
Immersion Oil	Type A	Type B	Type NVH	Type 300
Refractive Index F Line (486.1nm) e Line (546.1nm) D Line (589.3nm) C Line (656.3nm)	@23°C 1.5239 1.5180 1.5150 1.5115	@23°C 1.5236 1.5180 1.5150 1.5116	@23°C 1.5230 1.5178 1.5118 1.5118	@23°C 1.5238 1.5180 1.5150 1.5115
Dispersion: nF-nc: Abbe VD: Abbe Ve:	0.0124 41.6 41.2	0.0120 42.9 42.6	0.0112 46.0 45.7	0.0123 41.8 41.5
Temperature Coefficient: (15-35°C)-dnD/dt	0.00033	0.00031	0.00034	0.00033
Stability: (change in nd25°C after 24 hrs.@60°C) (@100°C)	0 0	0 0	0 0	0 0
Fluorescence: (1) Short wave: (Ultraviolet) Long Wave:	low low	low low	low low	low low
Color (Gardner):	1	1	1
Viscosity: cSt±1-% @23°C: (centistokes):	150 low	1250 high	21,000 very high	300 low
Density: @23°C gm/cc: US lb/gal:	.923 7.70	.923 7.70	.919 7.67	.923 7.73
Cloud Point:	0°C	0°C	0°C	<-13
Flash Point: (Cleveland Open Cup)	325°C	325°C	325°C	325°C
Neutralization No: (mgKOH/gm)	0.01 max.	0.01 max.	0.01 max.	0.01 max.

Why do Cargille Immersion Oils Meet the DIN 58 884?
(Deutsches Institut Für Normung e.V.)
Refractive index, dispersion and temperature standardizations are the key specifications for optical clarity and high resolution. (Viscosity and other physical properties are directed toward specific applications and convenience of the user.)
Immersion oil specifications must meet the design requirements of old instruments and become the design criteria for new instruments. Without these specifications, it would be much harder for the microscopist to find the proper oil for his or her particular instruments' requirements and optical imagery would suffer.
The specifications adopted by DIN resulted from their study of instrument requirements of many manufacturers. The proposed specifications were submitted for review, with Cargille Laboratories serving as liaison for the American manufacturers.
The present trend toward tighter specification for optical properties is under a program of the International Standards Organization (ISO).

Unique Applications - Special Properties
Because of widespread availability, consistent quality, and close specifications, Cargille Oils are used as:

a temporary mounting media
fluid mounts (permitting specimen rotation)
calibration liquids
optical coupling fluids
combination protective coating and examination fluid
transparency medium for translucent materials
filter clearing agent (as recommended by Millipore Corp.)

Important Comments
Cargille Immersion Oils are supplied as a microscopy accessory by American Optical and Bausch & Lomb, and accepted for use by all foreign microscope manufacturers. Cargille Immersion Oils are synthetic hydrocarbons, natural petroleum derivatives that contain no solvents and no PCB's. These oils are stable, non-drying, odorless, safe for lens and handling, and easily removable. MSDS sheets are available.

Ordering Information

Prod # Description Unit Price Order / Quote

19552
Cargille Immersion Oil, Type A, low viscosity, 118ml
each
$12.25

Qty:

U MSDS Low viscosity (150cs), low fluorescence, PCB-free.

Prod # Description Unit Price Order / Quote

19556
Cargille Immersion Oil, Type B, high viscosity, 118ml
each
$12.25

Qty:

U MSDS High viscosity (1250cs), low fluorescence, PCB-free.

Prod # Description Unit Price Order / Quote

19562
Cargille Immersion Oil, Type NVH, very high viscosity, 118ml
each
$15.25

Qty:

U MSDS Very high viscosity (21000cs), low fluorescence, PCB-free.

Prod # Description Unit Price Order / Quote

19564
Cargille Immersion Oil, Type DF, 1 oz. (29.5ml)
each
$9.20

Qty:

U MSDS Very high viscosity (21000cs), low fluorescence, PCB-free.

Prod # Description Unit Price Order / Quote

19568
cargille Immersion Oil, 5-Pack, 5 x 7.4cc 1ea. Type 300, 1ea. Type NVH, 2ea. Type A, 1ea. Type B
each
$13.30

Qty:

U Keeps all types handy (A, B, NVH and Type 300).

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Page Last Updated: October 31, 2007