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Embedding Kits

Embedding Chemicals for Electron Microscopy and Light Microscopy

[Araldite 502] [Eponate 12™] [Glycol Methacrylate] [JB-4^®]
[Low Viscosity "Spurr"][PELCO^® Acrylic Resin][Poly/Bed^® 812]
Test Kits:
[Poly/Bed^® Resin Test Kit] [Low Viscosity "Spurr" Test Kit]

Hazard Warning Guides Used in the Following Listings

Cg = Suspected Carcinogen

C = Corrosive

F = Flammable

I = Irritant

L = Lachrymator

O = Oxidizing

R = Radioactive

S = Sensitizer

T = Toxic

U = Unlisted as hazard; unknown hazard if any

An extra shipping charge is required by the Carriers when hazardous goods are transported.

Araldite 502 Embedding Kits

The Araldite 502 kit with BDMA accelerator is equally efficient as the same kit using DMP-30. BDMA has a lower viscosity and diffuses more rapidly into tissues, porous specimens and rough surfaces. The Araldite 502 resins have better beam stability than the Eponate resins. BDMA is used in 3% concentrations versus 1.5 to 2% for DMP-30. Araldite kit may be used alone or mixed with other epoxies. Curing times are 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave^®.

1 Glauert AM, Glauert RH: Araldite as an embedding medium for electron microscopy. J Biophys Biochem Cytology, 4:191-194, 1958.
2 Glauert AM: Fixation, dehydration, and embedding of biological specimens. Practical Methods in Elec Micr, 3(1). Glauert AM, ed., North-Holland, Amsterdam, 5th reprint 1975.

Prod # Description Unit Price Order / Quote

18052
Araldite 502 Kit, 950ml (w/BDMA)
each
$39.06

Qty:

Cg, l Araldite 502, 450ml (18060) MSDS

l DDSA, 450g (18022) MSDS

C, T, I, F BDMA, 25g (18241) MSDS

Prod # Description Unit Price Order / Quote

18050
Araldite 502 Kit, 950ml (w/DMP-30)
each
$42.00

Qty:

Cg, l Araldite 502, 450ml (18060) MSDS

l DDSA, 450g (18022) MSDS

C, I DMP-30, 25g (18042) MSDS

eponate 12 kit

Eponate 12™ Kit, with BDMA

Aliphatic epoxy resin based on di- and tri-glydicyl ethers of glycerol with a significantly lower viscosity compared to Araldite 502. BDMA (<3%, see notes preceding) is used as the accelerator since it has lower viscosity and diffuses into tissues more rapidly. (See references below).
The viscosity of Eponate™ 12 at 25ºC is 15-21cp, WPE is 140–145 and Specific Gravity is 1.15–1.22g/ml. Other kit viscosities at 25ºC include DDSA (290cp) and NMA (225-275cp). Eponate™ 12 is hygroscopic.
Curing time is 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave^®.

First use of Epon 812 in U.S.A.:
Finck H, 1960. Epoxy resins in electron microscopy. J Biophys Biochem Cytol, 7, p. 27.
Luft JH, 1961. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol, 9, p. 409.

Prod # Description Unit Price Order / Quote

18012
Eponate 12 Embedding Kit, with BDMA
each
$71.51

Qty:

PELCO^® Technical Notes, PELCO^® Eponate 12™ Kit with DMP-30 and BDMA (PDF File)

Cg, l Eponate 12 Resin, 450g (18005) MSDS

l DDSA, 450g (18022) MSDS

C, I NMA, 450g (18032) MSDS

C, T, I, F BDMA, 25g (18241) MSDS

Eponate 12™ Embedding Kit, with DMP-30

Prod # Description Unit Price Order / Quote

18010
Eponate 12 Kit (DMP-30 catalyst)
each
$73.04

Qty:

PELCO^® Technical Notes, PELCO^® Eponate 12™ Kit with DMP-30 and BDMA (PDF File)

l Eponate 12 Resin, 450g (18005) MSDS

l DDSA, 450g (18022) MSDS

C, I NMA, 450g (18032) MSDS

T DMP-30, 25g (18042) MSDS

NMA Hardener in increased amounts, in the embedding mixture, make a harder block.

Eponate 12™ –Araldite 502 Kit

Prod # Description Unit Price Order / Quote

18028
Eponate 12™ –Araldite 502 Kit
each
$67.82

Qty:

l Eponate 12™ Resin, 450g (18005) MSDS

T, l Araldite 502 Resin, 450g (18060) MSDS

I DDSA, 450g (18022) MSDS

C, T, I. F BDMA, 25g (18241) MSDS

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PELCO^® Technical Notes, PELCO^® Araldite 502 Kit with DMP-30 and BDMA (PDF File)

Glycol Methacrylate (GMA) Kit

Prod # Description Unit Price Order / Quote

18350
Glycol methacrylate (GMA) Kit
each
$57.50

Qty:

F, l, T Glycol Methacrylate (GMA), 450ml (18091) MSDS

F, l, T n-Butyl Methacrylate, 225ml (18035) MSDS

O Benzoyl Peroxide Paste (50%), 15g (18097) MSDS

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JB-4^® Embedding Kit

Based on Glycol Methacrylate (GMA) plastic embedding

Prod # Description Unit Price Order / Quote

18020
JB4^® Embedding Kit
each
$259.30

Qty:

F, I, T Solution A, 800ml (18023) MSDS

F, I, T Solution B, 30ml (18024) MSDS

O Catalyst, 12g (18025) MSDS

JB-4^® Plus Embedding Kit

JB-4 Plus^® Embedding Kit for light microscopy is a water soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. Its cooler acting accelerator system offers greater heat protection of tissue components. It yields semi-thin sections (0.5 - 2µm) with excellent morphological preservation. Crystal clear non-yellowing casts are obtained at room temperature in 90 minutes or less.

Prod # Description Unit Price Order / Quote

18040
JB4^® Plus Embedding Kit
each
$252.85

Qty:

F, I, T Monomer Solution A, 500ml (18043) MSDS

F, I, T Accelerator Solution B, 15ml (18044) MSDS

O Catalyst, 8g (18045) MSDS

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"Spurr" Low Viscosity Kits

ERL 4221 has replaced ERL 4206. ERL 4221 has a higher viscosity than the previously used ERL 4206.

Spurr AR, 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruc Res, 26, p. 31.

Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, V 14, No 4, July 2006

It gives excellent penetration for tough materials particularly in the studies of botanical, mineral and other hard biological specimens. When the mixture is kept at room temperature for 24 hours its viscosity will increase to 140cp, as is the case with other resins. When fully formulated, the Spurr’s resin will store in a deep-freeze for two months and at room temperature for two days. Curing is done at 70ºC for about 8 hours (see our Microwave system for significant time saving during epoxy processing).

For making high resolution replication molds for casting "Spurr" resin go to President Replication Kit

PELCO^® Technical Notes for Low Viscosity Kit (PDF 1.54MB)

Prod # Description Unit Price Order / Quote

18300-4221
Low Viscosity "Spurr" Kit
each
$62.40

Qty:

Cg ERL 4221 (replaces ERL 4206), 225ml (18306-4221) MSDS

I DER^® 736, 250g (18310) MSDS

I NSA, 450g (18301) MSDS

C DMAE, 25g (18315) MSDS

Prod # Description Unit Price Order / Quote

18108
Low Viscosity "Spurr" Mini Kit
each
$68.20

Qty:

Note: See Full Details for Prod. No. 18108
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Lowicryl^® Kit

MonoStep^® Lowicryl^® HM-20, Non-Polar

Prod # Description Unit Price Order / Quote

18174
MonoStep^® Lowicryl^® HM-20, Non-Polar, 225g
each
$226.50

Qty:

I, F MSDS

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PELCO^® Acrylic Embedding Resin Kit

A unique acrylic resin modeled after the UNICRYL^® acrylic resin. The advantage of this resin is that the catalyst is supplied separate from the resin components. Shelf life is improved and heat induced changes during shipping are eliminated.
This resin is ideally suited for LM and EM applications (immunocytochemistry, ultrastructural preservation, in situ hybridization with good cutting properties for either thick or thin sectioning). Unique cutting properties have been attributed to this resin formulation. It is felt that proteins, nucleic acids and macromolecules are revealed at the surface during sectioning. It is a hydrophilic resin which exhibits unique staining properties at the light level for a wide range of stains (i.e. Hemotoxylin, Silver Methenamine, Light Green, Eosin, Safranin, Toluidine Blue).
The resin contains Styrene Monomer (hazard, see MSDS), Monomeric (Meth)-acrylate Esters with Benzoyl Peroxide supplied as the initiator. Mixing of the initiator is required prior to use. The resin is best stored at -20°C after the addition of the initiator but the resin will remain liquid down to -50°C. It is a pale yellow liquid with a pungent odor that can be cured with either heat or UV radiation. Best results are attained when the resin is microwave cured. The resin should be capped during polymerization due to evaporation. Use proper gloves, fume hood and eye protection.

Prod # Description Unit Price Order / Quote

18190
PELCO¨ Acrylic Embedding Resin Kit, 500ml
each
$148.50

Qty:

F, I, T	PELCO^® Acrylic Embedding Resin Kit, 500ml MSDS
O, I	Benzoyl Peroxide Paste, 6.5g (18190-B) MSDS

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Poly/Bed^® 812 Embedding Kit, with DMP-30

Poly/Bed^® 812 for electron microscopy and histology for studies requiring beam stability, low shrinkage and good contrast. Epoxy formulations are lower in viscosity than Araldite formulations, and hence provide better penetration into tissue samples.

Prod # Description Unit Price Order / Quote

18109
Poly/Bed^® 812 Kit (w/DMP-30)
each
$173.90

Qty:

Cg, I POLY/EMBED 812, 500g

I Dodecenyl Succinic Anhydride (DDSA), 450g

C, I Nadic Methyl Anhydride (NMA), 450g

T DMP-30, 100g

Instructions included. back up